SpecificArabidopsis thalianamalic enzyme isoforms can provide anaplerotic pyruvate carboxylation function inSaccharomyces cerevisiae
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چکیده
منابع مشابه
A 13C mass isotopomer study of anaplerotic pyruvate carboxylation in perfused rat hearts.
Anaplerotic pyruvate carboxylation was examined in hearts perfused with physiological concentrations of glucose, [U-13C3]lactate, and [U-13C3]pyruvate. Also, a fatty acid, [1-13C]octanoate, or ketone bodies were added at concentrations providing acetyl-CoA at a rate resulting in either low or substantial pyruvate decarboxylation. Relative contributions of pyruvate and fatty acids to citrate syn...
متن کاملA C Mass Isotopomer Study of Anaplerotic Pyruvate Carboxylation in Perfused Rat Hearts*
Anaplerotic pyruvate carboxylation was examined in hearts perfused with physiological concentrations of glucose, [U-C3]lactate, and [UC3]pyruvate. Also, a fatty acid, [1-C]octanoate, or ketone bodies were added at concentrations providing acetyl-CoA at a rate resulting in either low or substantial pyruvate decarboxylation. Relative contributions of pyruvate and fatty acids to citrate synthesis ...
متن کاملPyruvate carboxylation as an anaplerotic mechanism in the isolated perfused rat heart.
1. The role of pyruvate carboxylation in the net synthesis of tricarboxylic acid-cycle intermediates during acetate metabolism was studied in isolated rat hearts perfused with [1-14C]pyruvate. 2. The incorporation of the 14C label from [1-14C]pyruvate into the tricarboxylic acid-cycle intermediates points to a carbon input from pyruvate via enzymes in addition to pyruvate dehydrogenase and citr...
متن کاملPhosphoenolpyruvate carboxykinase as the sole anaplerotic enzyme in Saccharomyces cerevisiae.
Pyruvate carboxylase is the sole anaplerotic enzyme in glucose-grown cultures of wild-type Saccharomyces cerevisiae. Pyruvate carboxylase-negative (Pyc(-)) S. cerevisiae strains cannot grow on glucose unless media are supplemented with C(4) compounds, such as aspartic acid. In several succinate-producing prokaryotes, phosphoenolpyruvate carboxykinase (PEPCK) fulfills this anaplerotic role. Howe...
متن کاملAnaplerotic role for cytosolic malic enzyme in engineered Saccharomyces cerevisiae strains.
Malic enzyme catalyzes the reversible oxidative decarboxylation of malate to pyruvate and CO(2). The Saccharomyces cerevisiae MAE1 gene encodes a mitochondrial malic enzyme whose proposed physiological roles are related to the oxidative, malate-decarboxylating reaction. Hitherto, the inability of pyruvate carboxylase-negative (Pyc(-)) S. cerevisiae strains to grow on glucose suggested that Mae1...
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ژورنال
عنوان ژورنال: The FEBS Journal
سال: 2017
ISSN: 1742-464X
DOI: 10.1111/febs.14013